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Reactivo de anticuerpo HIF-1α para inmunohistoquímica

Reactivo de anticuerpo HIF-1α para inmunohistoquímica

Anticuerpo IHC -- HIF-1 α


  • Control positivo:

    Breast cancer
  • Localización Celular:

    Cytoplasm/Nucleus
  • Solicitud:

    IHC-P
  • Anticuerpo secundario:

    iVision™
  • Nº de catálogo:

    AR0421
  • Especies anfitrionas:

    Rabbit
  • Clon:

    SD310
  • Especificación/ml:

    1、3、6、0.2(Concentrated)

Product name

IHC Antibody --HIF-1α


Packing specification

Code

Clone

Specifications

AR0421

SD310

0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml


Intended use

For Research use only. HIF-1α Antibody reagent is intended for use to qualitatively identify HIF-1α by microscopy in sections of FFPE tissue using immunohistochemical detection system.



Principle

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β. HIF-1α plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 



Main components

Immunoglobulin, antibody diluent


Storage

Store at 2~8℃ for 18 months


Sample requirements

FFPE tissues are usually cut into sections as thin as 35μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.


Protocol

1. Sample preparationDeparaffinize the slides in xylene , , Ⅲ for 5 minutesTransfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2BlockingBlock endogenous peroxidase activity by incubating sections in 3% H2O2 solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3Antigen retrievalHeat the EDTA Antigen retrieval buffer to 100. Then place the slides in the boiled buffer and continue to heat for 1520 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DABDrain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin stainingDrain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. DehydrateDehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.


Positive localization


1. Positive localization: cytoplasm/nuclear.

2. Positive control: breast cancer.



Precautions

1. Please read the instruction carefully and become familiar with all components of the kit prior to use, Strictly follow the instruction during operation.

2.  NO use el kit ni ningún componente del kit después de su.

3.  Solo los profesionales capacitados pueden usar este kit. Utilice una bata de laboratorio adecuada y guantes desechables mientras manipula los reactivos.

4.  Evite el contacto de la piel, los ojos y las membranas mucosas con los productos químicos.

5.  NO pipetear con la boca.

6.  Los reactivos no utilizados, el kit usado y los residuos deben eliminarse de acuerdo con las normativas locales.


Fabricante _

Nombre de la empresa: Xiamen Talent Biomedical Technology Co., Ltd

Dirección: Pisos n.º 3 y 4, edificio B10, n.º 2068 Wengjiao Road West, parque biomédico, distrito de Haicang, ciudad de Xiamen, 36100 China

Teléfono: +86 592 6315755            

Correo electrónico : tlsw@talentbiomedical.com                

Sitio web : www.talentbiomedical.com


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